Insect Ryanodine Receptor: Distinct but Coupled Insecticide Binding Sites for [N-C3H3]Chlorantraniliprole, Flubendiamide, and [3H]Ryanodine
نویسندگان
چکیده
منابع مشابه
Distinct ryanodine- and inositol 1,4,5-trisphosphate-binding sites in hepatic microsomes.
A light hepatic microsomal preparation was fractionated by sucrose-density centrifugation into one rough, one intermediate and two smooth fractions. The four fractions were characterized with respect to parameters relevant to Ca2+ sequestration. Ca2(+)-ATPase activity was similar in the rough, intermediate and smooth I fractions, but lower in the smooth II fraction. Ca2+ accumulation was the hi...
متن کاملDihydropyridine and Ryanodine Receptor Binding After
The purpose of this study was to determine if there are alterations in the dihydropyridine and/or ryanodine receptors that might explain the excitation-contraction uncoupling associated with eccentric contraction-induced skeletal muscle injury. The left anterior crural muscles (i.e., tibialis anterior, extensor digitorum longus, and extensor hallucis longus) of mice were injured in vivo by 150 ...
متن کاملS100A1 Protein Does Not Compete with Calmodulin for Ryanodine Receptor Binding but Structurally Alters the Ryanodine Receptor·Calmodulin Complex.
S100A1 has been suggested as a therapeutic agent to enhance myocyte Ca(2+) cycling in heart failure, but its molecular mode of action is poorly understood. Using FRET, we tested the hypothesis that S100A1 directly competes with calmodulin (CaM) for binding to intact, functional ryanodine receptors type I (RyR1) and II (RyR2) from skeletal and cardiac muscle, respectively. Our FRET readout provi...
متن کاملFunctional regulation of the cardiac ryanodine receptor by suramin and calmodulin involves multiple binding sites.
Suramin and structurally related compounds increase not only the open probability (P(o)) of ryanodine receptor (RyR) channels but also the single-channel conductance in a unique characteristic manner. In this report, we examine the mechanisms underlying the complex changes to cardiac RyR channel function caused by suramin and the evidence that these changes result from an interaction with calmo...
متن کاملAnti-ryanodine receptor antibody binding sites in vascular and endocardial endothelium.
The ryanodine receptor (RyR) functions as the calcium release channel of the sarcoplasmic reticulum activated by electromechanical coupling in skeletal and cardiac muscles. In smooth muscle, inositol trisphosphate releases calcium from internal stores during pharmacomechanical coupling, but these cells also contain ryanodine-sensitive calcium stores. In this study, we establish the presence of ...
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ژورنال
عنوان ژورنال: Chemical Research in Toxicology
سال: 2012
ISSN: 0893-228X,1520-5010
DOI: 10.1021/tx300326m